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In isolation stages, selection of the environment as a source of useful isolates is important. Seven hundred and eighteen strains were isolated on humic acid-vitamin agar and 220 strains were on methanol medium from three each paddy forest, field, riverside soil samples. So, during the screening of antibiotics from soil, We isolated 15 microorganism showing powerful antagonistic activity against Escherichia coli. Antimicrobial activity of three. Microorganism was tested against 25 strains of bacteria, yeast, fungus. Among them, No. 248 strain exhibited the most strongly growth inhibition. The property of No. 248 antimicrobial compound was very stable under acid(pH 3.0) and alkali(pH 10.0) treatment, but it was unstable in heat treatment at 120 C.
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